Guidance

Cycle threshold (Ct) in SARS-CoV-2 RT-PCR

A guide for health protection teams about interpreting Ct in real-time, reverse transcription polymerase chain reaction (RT-PCR) assays performed for Sars-CoV-2.

• From: Public Health England
• Published: 28 October 2020

Documents

Understanding cycle threshold (Ct) in SARS-CoV-2 RT-PCR: a guide for health protection teams

Ref: PHE publications gateway number: GW-1651

PDF, 806 KB, 12 pages

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Details

This document outlines how health protection teams should report and apply Ct values for clinical interpretation and actions only in cases where the linearity, limit of detection and standard quantification curves are assured.

Published 28 October 2020