An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens

• From: Department for International Development
• Published: 1 January 2005

• Document Type: Journal Article
• Theme: Health
• Authors: Traore, H., Thomson, L.M., Yesilkaya, H., Doig, C., Steingrimsdottir, H., Garcia, L., Forbes, K.J.

Abstract

A single-step, 5-min lysis method was investigated as a rapid technique to extract genomic DNA from mycobacteria for PCR detection of M. tuberculosis directly from clinical specimens. Of 67 smear-positive clinical specimens, 64 (95.5%) were positive by PCR after this rapid extraction method.

Citation

Journal of Microbiological Methods (2005) 63 (1) 95-98 [doi:10.1016/j.mimet.2005.03.005]

Links

An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens

Published 1 January 2005