Enhanced control of pathogenic SIVmac239 replication in macaques immunized with a plasmid IL12 and a DNA prime, viral vector boost vaccine regimen

• From: Department for International Development
• Published: 1 January 2011

• Document Type: Journal Article
• Theme: Health
• Authors: McDermott, A.B., Wilson, A.J., Parks, C.L., Boggiano, C. and Cassamasa, H.
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  Chiuchiolo, M.J., Coleman, J.W., Dean, H.J., Felber, B.K., Ginsberg, A.A., Hudgens, M.G., Kemelman, M., King, C.R., Lin, F., Lopez, M., McBride, D., Morrow, G., Mullen, K., Narpala, S., Ouellett, I., Pavlakis, G.N., Sardesai, N.Y., Schultz, A., Winstone, N., Wu, D., and Zamb, T.J.

Abstract

DNA priming has previously shown to elicit augmented immune responses when administered by electroporation (EP) or co-delivered with plasmid encoding IL12 (pIL-12). We hypothesized that the efficacy of a DNA prime and recombinant Adenovirus 5 boost vaccination regimen (DNA/rAd5) would be improved when incorporating these vaccination strategies into the DNA priming phase, as determined by pathogenic SIVmac239 challenge outcome. The whole SIVmac239 proteome was delivered in 5 separate DNA plasmids (pDNA-SIV) by EP with or without pIL12, followed by boosting 4 months later with corresponding rAd5-SIV vaccine vectors. Remarkably, after repeated low dose SIVmac239 mucosal challenge, we demonstrate a 2.6 and 4.4 log reduction of median SIV peak and set-point viral load in rhesus macaques (RMs) that received pDNA-SIV by EP with pIL12 compared to mock immunized controls (p

Citation

Journal of Virology (2011) [doi:10.1128/JVI.05060-11] JVI Accepts, published online ahead of print on 6 July 2011

Links

Enhanced control of pathogenic SIVmac239 replication in macaques immunized with a plasmid IL12 and a DNA prime, viral vector boost vaccine regimen

Published 1 January 2011