Isolation and expression of tilapia (Oreochromis niloticus) serine 8-type GnRH coding and regulatory sequences
Abstract
The complete Serine 8-type gonadotropin releasing hormone (GnRH) coding sequence with a substantial 5-prime regulatory sequence (5 kb) has been isolated and characterised in Nile Tilapia (Oreochromis niloticus) from a relevant genomic library. The primary structure of the protein precursor was identified for this gene. The promoter efficacy has been tested using 0.6 kb of the GnRH promoter driving a lacZ reporter gene in both cultured spleen cells and transiently expressing zebrafish. In the cell transfection experiments, the average level of β-galactosidase activity in transfected cells was more than 2.1 (P
Citation
Gene, (2003), vol. 304, pp. 97-106 [doi:10.1016/S0378-1119(02)01184-8]