Performance of the HerpeSelect (Focus) and Kalon enzyme-linked immunosorbent assays for detection of antibodies against Herpes simplex virus type 2 using monoclonal antibody-blocking enzyme immunoassay (MAb-EIA) and clinico-virological reference standards in Brazil

• From: Department for International Development
• Published: 1 January 2007

• Country: Brazil
• Document Type: Journal Article
• Theme: Health
• Authors: Mayaud, P., Nascimento, M.C., Pannuti, C.S., Ferreira, S., Sabino, E.C., Hamilton, I., Parry, J.

Abstract

A total of 586 serum samples were used to evaluate the performance of type-specific herpes simplex virus type 2 (HSV-2) commercial enzyme-linked immunosorbent assays (ELISAs) by using the monoclonal antibody-blocking enzyme immunoassay (MAb-EIA) and a clinicovirological panel as reference standards. The Kalon and HerpeSelect ELISAs had similar sensitivities (93.5% and 93.8% compared with the results obtained by MAb-EIA, respectively, and 100% for both ELISAs compared with the results obtained with a clinicovirological panel). The Kalon ELISA had a higher specificity (96.5% and 96.8% compared with the results obtained by MAb-EIA and with a clinicovirological panel, respectively) than the HerpeSelect ELISA (86.9% and 94% compared with the results obtained by MAb-EIA and with a clinicovirological panel, respectively). A higher cutoff significantly improved the specificity of the HerpeSelect ELISA.

Citation

Journal of Clinical Microbiology (2007) 45 (7) 2309-2311

Links

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Published 1 January 2007